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trans blot sd semidry electrophoretic transfer cell system  (Bio-Rad)


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    Bio-Rad trans blot sd semidry electrophoretic transfer cell system
    Trans Blot Sd Semidry Electrophoretic Transfer Cell System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semidry+electrophoretic+transfer+cell/Trans-Blot+SD/pm40715177-94-15-22
    Average 99 stars, based on 3980 article reviews
    trans blot sd semidry electrophoretic transfer cell system - by Bioz Stars, 2026-09
    99/100 stars

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    Electrophoresis:

    Article Title: Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.
    Article Snippet: .. After electrophoresis at 180 V for 1 h, transfer of separated bands onto nitrocellulose membrane was performed using a semidry electrophoretic transfer cell (Biorad) at 25 V, 1 A for 30 min. Anti-PilA antibody [14, 15] was used at a dilution of 1:5,000, followed by anti-rabbit HRP-conjugated secondary antibody at 1:5,000 dilution and detection of bands was done using Supersignal West Pico chemiluminescence substrate (Pierce), with exposure time of 330 s. Triplicates of synchronized cultures were used for each strain. .. Quantification of PilA band-intensity in pixels was performed for all three replicates of each strain using ImageJ software [45], and the average intensity and the standard deviation was calculated for each timepoint for each strain from all three replicates.

    Article Title: Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli
    Article Snippet: .. After electrophoresis, the proteins were transferred from the gels to PVDF membranes using a semidry electrophoretic transfer cell ( Bio-Rad ) at 15 V for 40 min. For the Western blot analysis, a monoclonal antibody directed against the Flag epitope ( Sigma-Aldrich ) diluted 1:10,000 in a solution of PBS, 0.2% Triton and 3% skim milk was used. ..

    Membrane:

    Article Title: Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.
    Article Snippet: .. After electrophoresis at 180 V for 1 h, transfer of separated bands onto nitrocellulose membrane was performed using a semidry electrophoretic transfer cell (Biorad) at 25 V, 1 A for 30 min. Anti-PilA antibody [14, 15] was used at a dilution of 1:5,000, followed by anti-rabbit HRP-conjugated secondary antibody at 1:5,000 dilution and detection of bands was done using Supersignal West Pico chemiluminescence substrate (Pierce), with exposure time of 330 s. Triplicates of synchronized cultures were used for each strain. .. Quantification of PilA band-intensity in pixels was performed for all three replicates of each strain using ImageJ software [45], and the average intensity and the standard deviation was calculated for each timepoint for each strain from all three replicates.

    Article Title: In the absence of UCP1-mediated diet-induced thermogenesis, obesity is augmented even in the obesity-resistant 129S mouse strain
    Article Snippet: Total protein concentration was determined with the Lowry method and spectrophotometry at λ = 750 nm (Beckman DU 530). .. Samples were diluted in sample buffer [50 mM Tris·HCl, pH 6.8, 2% (wt/vol) SDS, 50 mM dl -dithiothreitol (DTT), 10% glycerol, and 0.02% bromphenol blue] and boiled at 95°C for 5 min. Proteins were separated by SDS-PAGE on an 11–12% polyacrylamide gel and transferred to a PVDF membrane (Bio-Rad) using a semidry electrophoretic transfer cell (Bio-Rad Trans-Blot SD; Bio-Rad Laboratories). ..

    Article Title: Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry ( Fragaria vesca ) and Functional Study of FvChi-14 .
    Article Snippet: The gels were stained with InstaBlue protein stain solution (APE×BIO, Des Moines, IA, USA), and the results were observed with Gel Doc XR+ System (Bio-Rad, Hercules, CA, USA). .. Proteins were electroblotted onto an NC membrane using semidry electrophoretic transfer cell (BIORAD, USA) following separation on 12% SDS-PAGE. ..

    SDS Page:

    Article Title: Nonstructural Protein 1 of Variant PEDV Plays a Key Role in Escaping Replication Restriction by Complement C3.
    Article Snippet: .. The obtained protein samples were separated on SDS-PAGE gels and transferred onto 0.22-mm polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). .. The membranes were blocked with 5% (wt/vol) nonfat dry milk overnight at 4°C and then probed with anti-C3 (Invitrogen; catalog number PA5-21349; 1:1,000), C/EBP-b (ABclonal Technology; catalog number A19538; 1:1,000), P-C/EBP-b (ABclonal Technology; catalog number AP1055; 1:1,000), anti-p38-MAPK (Beyotime Biotechnology; catalog number AF7668; 1:1,000), P-p38-MAPK (Beyotime Biotechnology; catalog number AF5887; 1:1,000), anti-PEDV-N (made in lab; 1:1,000), or anti-HA tag, Flag tag, and b-actin (Proteintech Group; catalog numbers 66006-2-Ig, 66008-4-Ig, 66009-1-Ig; 1:20,000) primary antibodies at 37°C for 1 h. After thorough washing with PBS 1 0.1% (vol/vol) Tween 20 (PBST), the membranes were incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (Proteintech Group; 1:10,000) overnight at 4°C.

    Article Title: In the absence of UCP1-mediated diet-induced thermogenesis, obesity is augmented even in the obesity-resistant 129S mouse strain
    Article Snippet: Total protein concentration was determined with the Lowry method and spectrophotometry at λ = 750 nm (Beckman DU 530). .. Samples were diluted in sample buffer [50 mM Tris·HCl, pH 6.8, 2% (wt/vol) SDS, 50 mM dl -dithiothreitol (DTT), 10% glycerol, and 0.02% bromphenol blue] and boiled at 95°C for 5 min. Proteins were separated by SDS-PAGE on an 11–12% polyacrylamide gel and transferred to a PVDF membrane (Bio-Rad) using a semidry electrophoretic transfer cell (Bio-Rad Trans-Blot SD; Bio-Rad Laboratories). ..

    Article Title: Quantitative Proteomic Analysis of Porcine Intestinal Epithelial Cells Infected with Porcine Deltacoronavirus Using iTRAQ-Coupled LC-MS/MS
    Article Snippet: .. The resulting protein samples were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm poly(vinylidene fluoride) (PVDF) membranes (Millipore, Bedford, MA, USA) using a semidry electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA). ..

    Western Blot:

    Article Title: Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli
    Article Snippet: .. After electrophoresis, the proteins were transferred from the gels to PVDF membranes using a semidry electrophoretic transfer cell ( Bio-Rad ) at 15 V for 40 min. For the Western blot analysis, a monoclonal antibody directed against the Flag epitope ( Sigma-Aldrich ) diluted 1:10,000 in a solution of PBS, 0.2% Triton and 3% skim milk was used. ..

    FLAG-tag:

    Article Title: Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli
    Article Snippet: .. After electrophoresis, the proteins were transferred from the gels to PVDF membranes using a semidry electrophoretic transfer cell ( Bio-Rad ) at 15 V for 40 min. For the Western blot analysis, a monoclonal antibody directed against the Flag epitope ( Sigma-Aldrich ) diluted 1:10,000 in a solution of PBS, 0.2% Triton and 3% skim milk was used. ..



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